4/8/2024 0 Comments Western blot protein ladderFor example, the enhanced standard may be necessary when using a 1:50,000 (25 ng/mL) to 1:100,000 (10 ng/mL) dilution of secondary antibody. SuperSignal Enhanced Molecular Weight Protein ladder is formulated specifically for applications requiring mouse monoclonal antibodies and experiments where very dilute antibody concentrations are used. This formulation is appropriate for use with most rabbit and other non-mouse polyclonal antibodies. Two versions of the MW marker are offered. The protein markers can then be visualized using appropriate substrates for enzyme-labeled antibodies or via fluorescent dye-labeled antibodies (not recommended for antibodies labeled with fluors in the 500–550 nm channel). When separating proteins, pouring gels by hand is a time-consuming and error-prone challenge. The ladder’s recombinant proteins bind to the antibodies used in the western blot through the IgG binding site. Protein Staining, Protein Ladder, Precast Protein Gels and Electrophoresis Chamber. Comparison chart and selection guide for protein gel electrophoresis size standards, ladders, and molecular weight markers (MW markers), including unstained and prestained varieties for in-gel or western blot detection. What should I do Here are some suggestions: Make sure that the correct amount of standard is loaded per lane. Get more from your multiplex westerns Fluorescent. We offer optimized reagents, buffers and fluorescent conjugated antibodies so you can get the most from your fluorescent western blots. The protein standard is supplied in a ready-to-use format for direct loading onto gels no need to heat, reduce, or add sample buffer prior to use.Ĭompare and view all other protein standards and ladders › Fluorescent western blotting provides accurate, quantitative, stable signals, and the ability to clearly evaluate multiple protein targets on a single blot. Precision Plus Protein Unstained Recombinant Protein Standards are Strep-tagged, enabling immunodetection and molecular weight determination on western blots. Thermo Scientific SuperSignal Molecular Weight Protein Ladder (20 to 150K) is a mix of eight unstained recombinant proteins with IgG-binding sites for chemiluminescent, fluorescent, chromogenic or other detection systems. Unstained natural protein standards allow accurate MW determination with uniform band intensities on SDS-PAGE gels stained with Coomassie Blue or zinc.
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